亚洲日本精品国产第一区二区_香港经典a毛片免费观看hd_欧美放荡的少妇_亚洲va久久久噜噜噜久久_少妇装睡让我滑了进去

咨詢(xún)熱線(xiàn)

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>倉(cāng)鼠肺細(xì)胞>CRL-1657R 1610 倉(cāng)鼠肺細(xì)胞

R 1610 倉(cāng)鼠肺細(xì)胞

簡(jiǎn)要描述:CRL-1657 R 1610 倉(cāng)鼠肺細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號(hào):CRL-1657
  • 廠(chǎng)商性質(zhì):生產(chǎn)廠(chǎng)家
  • 更新時(shí)間:2024-11-14
  • 訪(fǎng)  問(wèn)  量:2073

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

CRL-1657 R 1610 倉(cāng)鼠肺細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件


CRL-1657 R 1610 倉(cāng)鼠肺細(xì)胞 的詳細(xì)介紹

CRL-1657

ATCC® Number:



Shipped:  frozen CRL-1657™

Designations:  R 1610

Depositors:   M Rosenberg

Biosafety Level: 1

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Cricetulus griseus (hamster, Chinese)

Morphology: fibroblast



Source: Organ: lung

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applications: transfection host

Gender:  male

Comments: This line is a clonal derivative of V79 cells (see also V79-4, ATCC CCL-93) that had been treated with ethylmethanesulfonate.

The cells are deficient in galactokinase and are resistant to 2-deoxygalactose and 8-azaguanine.

This is a Chinese hamster somatic cell line.

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.



Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Preservation:  Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liqid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

parental cell line:ATCC CCL-93

References: 10111: Schumperli D, et al. Efficient expression of Escherichia coli galactokinase gene in mammalian cells. Proc. Natl. Acad. Sci. USA 79: 257-261, 1982. PubMed: 6281760

25665: Thirion JP, et al. Galactokinase mutants of Chinese hamster somatic cells resistant to 2-deoxygalactose. Genetics 83: 137-147, 1976. PubMed: 1269916

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products






















產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話(huà):

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢(xún)熱線(xiàn)

400-821-8510

(周一至周日9:00- 19:00)

在線(xiàn)咨詢(xún)
  • 掃一掃 微信咨詢(xún)

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
久久精品国产亚洲不av麻豆| 大地资源在线播放观看mv| 两个人看的www视频免费完整版| 人妻 日韩精品 中文字幕| 精品一卡2卡三卡4卡乱码精品视频| 国内揄拍国内精品少妇国语| 亚洲精品无码国模| 午夜成人无码福利免费视频| 中文字幕在线精品视频入口一区| 隔壁老王国产在线精品| 蜜臀久久人妻一区二区| 精品人妻无码区二区三区| 国内精品久久久久伊人av | 久久99热人妻偷产国产| 久久久久久国产精品免费免费男同| 无码av大香线蕉| 国产精品国产三级国产试看| 久久精品九九亚洲精品| 久久精品国产www456c0m| 亚洲av无码一区二区二三区软件| 狼狼综合久久久久综合网| 国产成a人片在线观看视频下载| 欧美激情一区二区三区| 精品久久久久久无码专区| 国产色视频一区二区三区| 亚洲精品国产专区无套| 97色伦图片97综合影院| 一级做a爱视频在线播放| 琪琪色原网站在线观看| 真正免费毛片在线播放| 日本道专区无码中文字幕| 亚洲欧美一区二区三区日产| 国产精品久久中文字幕| 奇米777四色影视在线看| 国产自产精品青青草原| 婷婷精品国产亚洲av麻豆不片 | 99精品国产兔费观看久久99| 国产婷婷成人久久av免费高清 | 国产二级一片内射视频插放 | 国产精品99久久99久久久动漫| 88国产精品视频一区二区三区|